3AGH
X-ray analysis of lysozyme in the presence of 200 mM Arg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-13 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.299, 78.299, 37.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.150 - 1.490 |
| R-factor | 0.1762 |
| Rwork | 0.175 |
| R-free | 0.20459 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a34 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.593 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.034 | 0.130 |
| Number of reflections | 18454 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 15.2 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | 0.05M Sodium Acetate, 1.6M Sodium Chloride, 0.2M Arg, pH 4.5, VAPOR DIFFUSION, temperature 293K |
