3AG3

Bovine Heart Cytochrome c Oxidase in the Nitric Oxide-bound Fully Reduced State at 100 K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Collection date	2007-12-12
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	182.289, 208.358, 177.916
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
Rwork	0.175
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2eij
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	83.600
High resolution limit [Å]	1.800
Number of reflections	617758

Crystallization Conditions

crystal ID	method	pH	temperature	details
1