3AFJ
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-01 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.137, 97.963, 104.228 |
Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
Resolution | 27.870 - 1.900 |
R-factor | 0.1359 |
Rwork | 0.133 |
R-free | 0.18607 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cqt |
RMSD bond length | 0.024 |
RMSD bond angle | 1.830 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.103 | 0.425 |
Number of reflections | 129821 | |
<I/σ(I)> | 13.6 | 3 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 277 | Na/K phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |