3AFJ
Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.137, 97.963, 104.228 |
| Unit cell angles | 90.00, 102.67, 90.00 |
Refinement procedure
| Resolution | 27.870 - 1.900 |
| R-factor | 0.1359 |
| Rwork | 0.133 |
| R-free | 0.18607 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cqt |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.103 | 0.425 |
| Number of reflections | 129821 | |
| <I/σ(I)> | 13.6 | 3 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 277 | Na/K phosphate, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
