3AFG
Crystal structure of ProN-Tk-SP from Thermococcus kodakaraensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.009, 123.996, 141.037 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.170 - 2.000 |
| R-factor | 0.18431 |
| Rwork | 0.181 |
| R-free | 0.24007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1scj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.227 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.113 | 0.365 |
| Number of reflections | 74932 | |
| <I/σ(I)> | 13.1 | 2.6 |
| Completeness [%] | 99.0 | 95.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES-NaOH (pH 6.5), 5% (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
