3AFC
Mouse Semaphorin 6A extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.474, 89.060, 95.523 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 44.540 - 2.500 |
| R-factor | 0.21467 |
| Rwork | 0.211 |
| R-free | 0.27769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1olz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.540 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.389 |
| Number of reflections | 40593 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 99.8 | 99.2 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 22-24% (wt/vol) PEG 1500, 0.1M Tris-Cl pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
