3AES
Structure of the light-independent protochlorophyllide reductase catalyzing a key reduction for greening in the dark
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97931, 0.97950, 0.96426, 0.98338 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.302, 80.920, 176.235 |
Unit cell angles | 90.00, 100.87, 90.00 |
Refinement procedure
Resolution | 34.750 - 2.500 |
R-factor | 0.21 |
Rwork | 0.208 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.351 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.077 |
Number of reflections | 78168 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG3350, 0.2M AMMONIUM CLORIDE, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |