3AER
Structure of the light-independent protochlorophyllide reductase catalyzing a key reduction for greening in the dark
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-15 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.418, 80.941, 176.728 |
| Unit cell angles | 90.00, 100.66, 90.00 |
Refinement procedure
| Resolution | 47.090 - 2.800 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ZMP |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.448 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.106 |
| Number of reflections | 51405 |
| Completeness [%] | 92.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 20% PEG3350, 0.2M AMMONIUM CLORIDE, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
