3AEI
Crystal structure of the prefoldin beta2 subunit from Thermococcus strain KS-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-11-17 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 67.315, 67.315, 92.415 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.660 - 1.700 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.24000 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Total number of observations | 307371 |
Number of reflections | 24056 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 500mM ammonium sulfate, 500mM ammonium chloride, 100mM sodium citrate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 500mM ammonium sulfate, 500mM ammonium chloride, 100mM sodium citrate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |