3ADY
Crystal structure of DotD from Legionella
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-11-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97897 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.860, 103.860, 103.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.720 - 2.000 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.24700 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.410 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 12739 | |
<I/σ(I)> | 19.9 | 5.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.1 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | 8% PEG 8000, 0.1M CHES-NaOH, pH 10.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |