3ADO

Crystal Structure of the Rabbit L-Gulonate 3-Dehydrogenase

Replaces:  2DPO

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2005-06-21
Detector	RIGAKU RAXIS V
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	71.809, 69.079, 65.642
Unit cell angles	90.00, 102.73, 90.00

Refinement procedure

Resolution	28.960 - 1.700
R-factor	0.183
Rwork	0.182
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hdh
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.075	0.537
Number of reflections	34467
<I/σ(I)>	9	3.3
Completeness [%]	100.0	99.9
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.6	291	ammonium acetate, sodium acetate trihydrate, polyethylene glycol 4000, glycerol anhydrous, pH 4.6, MICROBATCH, temperature 291K