3ADL

Structure of TRBP2 and its molecule implications for miRNA processing

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	I 21 21 21
Unit cell lengths	55.300, 60.425, 99.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.840 - 2.200
R-factor	0.26267
Rwork	0.261
R-free	0.29836
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1di2
RMSD bond length	0.012
RMSD bond angle	1.492
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	8276
<I/σ(I)>	54	10.1
Completeness [%]	99.1
Redundancy	14.4	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	AS, MgSO4, Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K