3ACP

Crystal Structure of Yeast Rpn14, a Chaperone of the 19S Regulatory Particle of the Proteasome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-13
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 64
Unit cell lengths	78.581, 78.581, 110.122
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.340 - 2.000
R-factor	0.203
Rwork	0.200
R-free	0.25500
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.968
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.280	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.046	0.405
Total number of observations	158070
Number of reflections	26081
<I/σ(I)>	10.1	2.1
Completeness [%]	100.0	100
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 3350, MgCl2, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
2