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3ACC

Crystal structure of hypoxanthine-guanine phosphoribosyltransferase with GMP from Thermus thermophilus HB8

Replaces:  2YWT
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSPRING-8 BEAMLINE BL26B2
Synchrotron siteSPring-8
BeamlineBL26B2
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2007-03-18
DetectorRIGAKU RAXIS V
Wavelength(s)1.0000
Spacegroup nameP 65 2 2
Unit cell lengths67.139, 67.139, 152.543
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution46.240 - 2.160
R-factor0.21
Rwork0.210
R-free0.25000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3acb
RMSD bond length0.006
RMSD bond angle1.300
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareMOLREP
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.240
High resolution limit [Å]2.1602.160
Rmerge0.0860.163
Number of reflections10796
Completeness [%]93.486.1
Redundancy10.29
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP829315% Glycerol, 28% Dioxane, 0.005M GMP, 0.01M Magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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