3ACC
Crystal structure of hypoxanthine-guanine phosphoribosyltransferase with GMP from Thermus thermophilus HB8
Replaces: 2YWTExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-18 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 67.139, 67.139, 152.543 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.240 - 2.160 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3acb |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.160 | 2.160 |
Rmerge | 0.086 | 0.163 |
Number of reflections | 10796 | |
Completeness [%] | 93.4 | 86.1 |
Redundancy | 10.2 | 9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15% Glycerol, 28% Dioxane, 0.005M GMP, 0.01M Magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |