3ACB
Crystal structure of hypoxanthine-guanine phosphoribosyltransferase from Thermus thermophilus HB8
Replaces: 2YWSExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-18 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 66.712, 66.712, 152.557 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.060 - 2.060 |
R-factor | 0.201 |
Rwork | 0.201 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yfz |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.130 |
High resolution limit [Å] | 2.060 | 2.060 |
Rmerge | 0.088 | 0.319 |
Number of reflections | 12932 | |
Completeness [%] | 99.8 | 98.3 |
Redundancy | 10.8 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 15% Glycerol, 38% Dioxane, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |