3AC0
Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97831, 0.97928, 0.96405 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 245.847, 148.412, 119.642 |
| Unit cell angles | 90.00, 112.84, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.540 |
| R-factor | 0.17292 |
| Rwork | 0.169 |
| R-free | 0.24241 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.692 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.540 | 2.550 |
| Rmerge | 0.089 | 0.440 |
| Number of reflections | 129736 | |
| <I/σ(I)> | 16.6 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | potassium dihydrogen phosphate, PEG 8000, glycerol, glucose, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
