3ABZ
Crystal structure of Se-Met labeled Beta-glucosidase from Kluyveromyces marxianus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-03 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.97831, 0.97928, 0.96405 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 245.678, 148.639, 119.779 |
Unit cell angles | 90.00, 112.95, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.150 |
R-factor | 0.16295 |
Rwork | 0.160 |
R-free | 0.21762 |
Structure solution method | MAD |
RMSD bond length | 0.022 |
RMSD bond angle | 1.755 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.072 | 0.298 |
Number of reflections | 213699 | |
<I/σ(I)> | 20.1 | 3.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 293 | potassium dihydrogen phosphate, PEG 8000, glycerol, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K |