3ABH
Crystal structure of the EFC/F-BAR domain of human PACSIN2/Syndapin II (2.0 A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.522, 86.145, 353.051 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.590 - 2.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of the EFC/F-BAR domain of human PACSIN2 (AMINO ACIDS 1-343) |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 64396 | |
| <I/σ(I)> | 12.4 | 4.9 |
| Completeness [%] | 95.4 | 96 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG 3350, 0.1M ammonium fluoride, 0.01M Tris-HCl, 0.075M NaCl, 0.001M DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
