3AB8
Crystal Structure of the Hypothetical Tandem-type Universal Stress Protein TTHA0350 complexed with ATPs.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-02 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.910, 53.774, 63.429 |
| Unit cell angles | 78.91, 72.12, 69.66 |
Refinement procedure
| Resolution | 36.820 - 1.700 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ab7 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.820 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 47774 | |
| Completeness [%] | 95.1 | 90.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 8.6mM ATP, 30mM Magnesium chloride, 40% 1,2-propanediol, 100mM sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
