3AB1
Crystal Structure of Ferredoxin NADP+ Oxidoreductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9831 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.514, 127.971, 128.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.570 - 2.390 |
R-factor | 0.24232 |
Rwork | 0.240 |
R-free | 0.28891 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.390 | 2.400 |
Rmerge | 0.080 | 0.485 |
Number of reflections | 31262 | |
<I/σ(I)> | 12 | 3.92 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | 16 % PEG 6000, 0.1M ammonium sulfate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
2 |