3AAL
Crystal Structure of endonuclease IV from Geobacillus kaustophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2007-09-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.000000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 85.170, 85.170, 140.220 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.420 - 1.600 |
Rwork | 0.197 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xp3 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.660 |
High resolution limit [Å] | 1.600 | 3.450 | 1.600 |
Rmerge | 0.049 | 0.043 | 0.216 |
Number of reflections | 40267 | ||
<I/σ(I)> | 64.904 | ||
Completeness [%] | 99.7 | 99.4 | 97.6 |
Redundancy | 14.9 | 17.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M cacodylate, 30% polyethylene glycol 600, 1M NaCl, 10% glycerol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |