3AAD

Structure of the histone chaperone CIA/ASF1-double bromodomain complex linking histone modifications and site-specific histone eviction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-11
Detector	ADSC QUANTUM 210
Spacegroup name	P 61 2 2
Unit cell lengths	102.120, 102.120, 271.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.650 - 3.300
R-factor	0.24
Rwork	0.237
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.524
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.180	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.056	0.517
Number of reflections	17814
<I/σ(I)>	20.9	6.3
Completeness [%]	99.2	99.8
Redundancy	12.5	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.65	293	0.1M MES, 1.1M AMMONIUM SULFATE, 0.25M LITHIUM SULFATE, pH 5.65, VAPOR DIFFUSION, HANGING DROP, temperature 293K