3AAC
Small heat shock protein hsp14.0 with the mutations of I120F and I122F in the form II crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-21 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 41 |
| Unit cell lengths | 69.564, 69.564, 49.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.970 - 2.400 |
| Rwork | 0.245 |
| R-free | 0.27500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.494 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 8995 | |
| <I/σ(I)> | 16.1 | 2.7 |
| Completeness [%] | 95.0 | 88.7 |
| Redundancy | 4.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 290 | 40% ethylene glycol, 200mM zinc acetate, 100mM MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
