3AAB
Small heat shock protein hsp14.0 with the mutations of I120F and I122F in the form I crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-21 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 40.366, 61.096, 96.136 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.778 - 1.851 |
Rwork | 0.228 |
R-free | 0.23800 |
Structure solution method | MIRAS |
RMSD bond length | 0.004 |
RMSD bond angle | 0.628 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 20341 | |
<I/σ(I)> | 37.1 | 3 |
Completeness [%] | 96.8 | 92.3 |
Redundancy | 5.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 5% isopropanol, 10mM magnesium chloride, 50 mM Tris-HCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |