3AA8
Crystal Structure Analysis of the Mutant CutA1 (S11V/E61V) from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 61 |
Unit cell lengths | 52.664, 52.664, 178.361 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.670 - 2.300 |
R-factor | 0.174 |
Rwork | 0.174 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1naq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.117 | 0.330 |
Number of reflections | 12336 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.6 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Bis-Tris (pH 7.0), 1.2M tri-sodium citrate dihydrate, VAPOR DIFFUSION, SITTING DROP, temperature 20.0K, temperature 293K |