3AA4

A52V E.coli RNase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	41.472, 85.529, 35.143
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.760 - 1.790
R-factor	0.19361
Rwork	0.192
R-free	0.22125
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.191
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.760
High resolution limit [Å]	1.790
Number of reflections	12360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1