3AA3

A52L E. coli RNase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	43.715, 86.037, 34.901
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.020 - 2.200
R-factor	0.20563
Rwork	0.204
R-free	0.25163
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.873
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	43.020
High resolution limit [Å]	2.200
Number of reflections	6074

Crystallization Conditions

crystal ID	method	pH	temperature	details
1