3AA2

A52I E. coli RNase HI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Spacegroup name	P 21 21 21
Unit cell lengths	41.368, 85.301, 36.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.650 - 1.900
R-factor	0.20225
Rwork	0.201
R-free	0.23058
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.944
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.650
High resolution limit [Å]	1.900
Number of reflections	10201

Crystallization Conditions

crystal ID	method	pH	temperature	details
1