3A9Q
Crystal Structure Analysis of E173A variant of the soybean ferritin SFER4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2009-06-30 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 223.503, 221.816, 122.725 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.674 - 1.896 |
R-factor | 0.1522 |
Rwork | 0.150 |
R-free | 0.18500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3a68 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.870 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.896 | 1.900 |
Rmerge | 0.102 | 0.356 |
Number of reflections | 473818 | |
<I/σ(I)> | 15.7 | 8.34 |
Completeness [%] | 99.2 | 97 |
Redundancy | 6.6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 15% PEG 1000, 0.2M calcium acetate, 0.1M imidazole (pH 7.8) , VAPOR DIFFUSION, HANGING DROP, temperature 293K |