3A9Q
Crystal Structure Analysis of E173A variant of the soybean ferritin SFER4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-30 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 223.503, 221.816, 122.725 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.674 - 1.896 |
| R-factor | 0.1522 |
| Rwork | 0.150 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a68 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.870 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.896 | 1.900 |
| Rmerge | 0.102 | 0.356 |
| Number of reflections | 473818 | |
| <I/σ(I)> | 15.7 | 8.34 |
| Completeness [%] | 99.2 | 97 |
| Redundancy | 6.6 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 15% PEG 1000, 0.2M calcium acetate, 0.1M imidazole (pH 7.8) , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
