3A9L
Structure of Bacteriophage poly-gamma-glutamate hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2009-03-12 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.96400 |
Spacegroup name | P 32 |
Unit cell lengths | 86.809, 86.809, 85.116 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.040 - 1.900 |
R-factor | 0.28291 |
Rwork | 0.281 |
R-free | 0.31495 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.040 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.130 | 0.410 |
Number of reflections | 56281 | |
<I/σ(I)> | 15.19 | 7.69 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 10.1 | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 40%(v/v) PEG 300, 5%(w/v) PEG 1000, 0.1M Tris buffer pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |