3A9F
Crystal structure of the C-terminal domain of cytochrome cz from Chlorobium tepidum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 74.619, 74.619, 111.211 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.320 - 1.300 |
| R-factor | 0.13891 |
| Rwork | 0.138 |
| R-free | 0.15454 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | OASIS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.048 | 0.198 |
| Number of reflections | 73534 | |
| <I/σ(I)> | 71.5 | 4.4 |
| Completeness [%] | 98.4 | 94.8 |
| Redundancy | 7.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 1.4M ammonium sulfate, 2% PEG 400, 0.1M sodium citrate pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
