3A96
Crystal structure of hen egg white lysozyme soaked with 100mM RhCl3 at pH2.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-12-02 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.967, 77.967, 37.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.380 - 1.750 |
R-factor | 0.19526 |
Rwork | 0.194 |
R-free | 0.22450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.012 |
RMSD bond angle | 1.357 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.065 | 0.325 |
Number of reflections | 11665 | |
Completeness [%] | 96.1 | 98 |
Redundancy | 21.5 | 21.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | sodium chloride, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |