3A8U
Crystal Structure of omega-Amino Acid:Pyruvate Aminotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-12 |
| Detector | RIGAKU |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 124.460, 137.790, 61.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.14675 |
| Rwork | 0.146 |
| R-free | 0.15763 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.341 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.900 | 1.190 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.032 | 0.773 |
| Number of reflections | 135011 | |
| <I/σ(I)> | 21 | 0.26 |
| Completeness [%] | 73.6 | 17.6 |
| Redundancy | 13 | 17.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Dialysis | 7.8 | 277 | 2.2M ammonium sulphate, 0.33M potassium phosphate buffer, pH 7.8, Dialysis, temperature 277K |
