3A8R
The structure of the N-terminal regulatory domain of a plant NADPH oxidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2006-04-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.418, 72.162, 118.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.470 - 2.400 |
R-factor | 0.235 |
Rwork | 0.234 |
R-free | 0.26600 |
Structure solution method | MAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.6.0041) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.048 | 0.234 |
Number of reflections | 20590 | |
<I/σ(I)> | 16.3 | 6 |
Completeness [%] | 97.7 | 86 |
Redundancy | 6.7 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES-NaOH buffer, pH 7.0 containing 0.6M sodium thiocyanate, 13%(w/v) PEG 8000, 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |