3A8P
Crystal structure of the Tiam2 PHCCEx domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.690, 104.790, 115.970 |
| Unit cell angles | 90.00, 80.55, 90.00 |
Refinement procedure
| Resolution | 29.810 - 2.100 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a8q |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 64571 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 97.6 | 80.6 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG 8000, 100mM Tris-HCl, 200mM Magnesium chloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
