3A7P

The crystal structure of Saccharomyces cerevisiae Atg16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	95
Detector technology	CCD
Collection date	2009-04-08
Detector	RAYONIX MX-225
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	114.191, 114.191, 156.286
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.100 - 2.800
R-factor	0.254
Rwork	0.253
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a7o
RMSD bond length	0.008
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.077	0.300
Number of reflections	15085
<I/σ(I)>	13.1
Completeness [%]	97.5	81
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	0.5M calcium chloride, 0.1M sodium acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K