3A7M
Structure of FliT, the flagellar type III chaperone for FliD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 35 |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 121.340, 121.340, 58.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.190 - 3.200 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.28600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.200 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 8390 | |
| <I/σ(I)> | 11.1 | 2.6 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 3.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | 1M Potassium sodium tartrate, 0.1M CHES, 0.2M Li2SO4, pH9.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
