3A7L
Crystal structure of E. coli apoH-protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-16 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 46.483, 52.037, 46.831 |
Unit cell angles | 90.00, 93.05, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.300 |
R-factor | 0.17784 |
Rwork | 0.176 |
R-free | 0.20513 |
Starting model (for MR) | 1htp |
RMSD bond length | 0.011 |
RMSD bond angle | 1.234 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.053 | 0.473 |
Number of reflections | 26800 | |
<I/σ(I)> | 32.5 | 3.1 |
Completeness [%] | 97.9 | 96.9 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 2.3M ammonium sulfate, 0.1M sodium citrate, 3%(v/v) 2-methyl-2,4-pentanediol, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |