3A71
High resolution structure of Penicillium chrysogenum alpha-L-arabinanase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9794, 0.9700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.877, 77.248, 79.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.140 |
| R-factor | 0.1067 |
| R-free | 0.12830 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.110 | 0.342 |
| Number of reflections | 149407 | |
| <I/σ(I)> | 32 | 5.4 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 7.2 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 277 | 28% MPD, 0.1M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
