3A62
Crystal structure of phosphorylated p70S6K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 68.742, 68.742, 144.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.010 - 2.350 |
| R-factor | 0.229 |
| Rwork | 0.229 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a60 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.010 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 15108 | |
| <I/σ(I)> | 25 | 8.19 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 10.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 0.1M Tris, pH 8.5, 3.5M Sodium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K |
