3A60
Crystal structure of unphosphorylated p70S6K1 (Form I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.600, 62.893, 86.992 |
| Unit cell angles | 90.00, 94.32, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.800 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | p70S6K1 homology model based on the published RSK1 structure; PDB ENTRY 2Z7R |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 20565 | |
| <I/σ(I)> | 14.3 | 2.85 |
| Completeness [%] | 97.9 | 83.8 |
| Redundancy | 3.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Bis-Tris, pH 5.5, 0.2M lithium sulfate, 22.5% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
