3A5V
Crystal structure of alpha-galactosidase I from Mortierella vinacea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-08 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0313 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 142.470, 142.470, 130.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.160 - 2.000 |
R-factor | 0.1433 |
Rwork | 0.142 |
R-free | 0.17008 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uas |
RMSD bond length | 0.014 |
RMSD bond angle | 1.291 |
Data reduction software | DPS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 | 0.343 |
Number of reflections | 45399 | |
<I/σ(I)> | 8.4 | 2.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 7.7 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2M trisodium citrate dihydrate, 0.1M Tris buffer pH 8.5, 30% polyethylene glycol 400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |