3A5T

Crystal structure of MafG-DNA complex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-14
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.97939, 0.97898, 0.96416
Spacegroup name	C 2 2 21
Unit cell lengths	40.364, 185.243, 230.263
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.080 - 2.800
Rwork	0.261
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.080	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.072	0.192
Total number of observations	284413
Number of reflections	22101
<I/σ(I)>	56.77	11.91
Completeness [%]	98.9	92.6
Redundancy	13	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	0.1M sodium acetate, 40mM magnesium chloride hexahydrate, 8% 2-methyl-2,4-pentandiol, 4mM Tris(2-carboxyethyl)phosphine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K