3A5T
Crystal structure of MafG-DNA complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-14 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97939, 0.97898, 0.96416 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 40.364, 185.243, 230.263 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.080 - 2.800 |
| Rwork | 0.261 |
| R-free | 0.29400 |
| Structure solution method | MAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.080 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.072 | 0.192 |
| Total number of observations | 284413 | |
| Number of reflections | 22101 | |
| <I/σ(I)> | 56.77 | 11.91 |
| Completeness [%] | 98.9 | 92.6 |
| Redundancy | 13 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M sodium acetate, 40mM magnesium chloride hexahydrate, 8% 2-methyl-2,4-pentandiol, 4mM Tris(2-carboxyethyl)phosphine, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
