3A5P

Crystal structure of hemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-09-08
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.502
Spacegroup name	P 21 21 21
Unit cell lengths	64.929, 77.980, 86.109
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.370 - 1.820
R-factor	0.156
Rwork	0.154
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	initial model determined by SAD
RMSD bond length	0.013
RMSD bond angle	1.244
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.820	1.820
Number of reflections	40006
<I/σ(I)>	49.4	11
Completeness [%]	99.8	100
Redundancy	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	vapour diffusion, hanging drop	6.5	293	15% PEG3350, 150mM NaI, pH 6.5, vapour diffusion, hanging drop, temperature 293K