3A5K
Crystal structure of protein-tyrosine phosphatase 1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2008-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.094, 60.755, 87.964 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.510 - 1.850 |
| R-factor | 0.19126 |
| Rwork | 0.189 |
| R-free | 0.23323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cma |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.991 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.052 | 0.028 | 0.270 |
| Number of reflections | 27426 | ||
| <I/σ(I)> | 49.639 | ||
| Completeness [%] | 98.6 | 99.5 | 88.8 |
| Redundancy | 13.3 | 13.5 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
