3A5J
Crystal structure of protein-tyrosine phosphatase 1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2008-11-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.426, 88.426, 72.467 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.880 - 1.700 |
| R-factor | 0.18995 |
| Rwork | 0.188 |
| R-free | 0.22857 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cma |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.180 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.051 | 0.252 |
| Number of reflections | 36101 | |
| <I/σ(I)> | 38.4 | 7.75 |
| Completeness [%] | 98.9 | 93.6 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
