3A5I
Structure of the cytoplasmic domain of FlhA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 41 |
| Unit cell lengths | 216.323, 216.323, 65.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.800 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.084 | 0.398 |
| Number of reflections | 37317 | |
| <I/σ(I)> | 13.8 | 3 |
| Completeness [%] | 99.8 | 98.8 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 10-15% PEG300, 4-6% PEG8000, 0.1M Tris-HCl, 0.5mM EDTA, 10-15% glycerol, pH8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 2 |
