3A5G
Crystal structure of a hemoglobin component V from Propsilocerus akamusi (pH7.0 coordinates)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-01-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.510, 64.140, 72.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.330 - 1.810 |
R-factor | 0.191 |
Rwork | 0.190 |
R-free | 0.20800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x3k |
RMSD bond length | 0.019 |
RMSD bond angle | 1.700 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | CNX (2005) |
Refinement software | CNX (2005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.080 | 1.870 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.054 | 0.229 |
Number of reflections | 14864 | |
<I/σ(I)> | 6.1 | |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 6.71 | 6.62 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |