3A5C
Inter-subunit interaction and quaternary rearrangement defined by the central stalk of prokaryotic V1-ATPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Collection date | 2009-03-05 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 381.578, 381.578, 147.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.920 - 4.510 |
| Rwork | 0.429 |
| R-free | 0.43700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3a5d |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 70598 | |
| <I/σ(I)> | 10.3 | 2.3 |
| Completeness [%] | 96.9 | 92.7 |
| Redundancy | 5.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 1.6M ammonium sulfate, 0.1M MES-NaOH (pH6.0), 10% dioxane, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
