3A5B
Crystal structure of a hemoglobin component V from Propsilocerus akamusi (pH6.5 coordinates)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.340, 63.610, 72.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.020 - 1.810 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x3k |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.100 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX (2005) |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 1.870 |
| High resolution limit [Å] | 1.810 | 1.810 |
| Rmerge | 0.062 | 0.198 |
| Number of reflections | 14451 | |
| <I/σ(I)> | 9.7 | |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 13.7 | 13.38 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
