3A59
Structure of Hemoglobin from flightless bird (Struthio camelus)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 291 |
Detector technology | IMAGE PLATE |
Collection date | 2009-05-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 82.051, 82.051, 214.928 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.750 - 3.410 |
R-factor | 0.2748 |
Rwork | 0.265 |
R-free | 0.35371 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fs4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.940 |
Data reduction software | AUTOMAR |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0066) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.750 | 3.540 |
High resolution limit [Å] | 3.410 | 3.410 |
Rmerge | 0.151 | |
Total number of observations | 43272 | |
Number of reflections | 13030 | |
<I/σ(I)> | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 60% Poly ethylene glycol 3350, 50mM Sodium phosphate buffer pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |